Use of heteroaryl-3-oxo-propanenitrile derivatives in treating clinical wherein myelopoiesis suppression occurs

ABSTRACT

Heteroaryl-3-oxo-propanenitrile derivatives of formula (I) ##STR1## wherein X represents an oxygen atom or a --CH(R 4 )--, --O--CH(R 4 )--,--S(O) n  --, --S(O) n  --CH(R 4 )--, --CH(R 4 )--O--, --CH(R 4 )--S(O) n  -- or --CH(R 4 )--CH 2  -- group wherein n is 0, 1 or 2; R 1  represents C 1  --C 6  alkyl, pyridyl or unsubstituted or substituted phenyl; R 2 , R 3  and R 4  are as herein defined; and Q is hydrogen, carboxy, C 2  -C 7  - alkoxycarbonyl or a --CON(R a )R b  group, R a  and R b  being as defined herein; and their pharmaceutically acceptable salts are useful in stimulating myelopoiesis in bone marrow suppressed mammals.

Suppression of myelopoiesis is a frequent and sometimes life-threatening side-effect of the chemo- and radio-therapy in cancer and of the drugs used to treat rheumatoid arthritis and other autoimmune diseases.

Suppression of myelopoiesis is common also in patients with severe burns and bone marrow disorders and in subjects that undergo organ transplantation, who are given immuosuppressant drugs to minimize the danger of rejection.

To find a drug which is effective in stimulating myelopoiesis in bone marrow suppressed patients still remains an important target for the today's Medicinal Chemistry, with large potential clinical application.

The present invention relates, in one aspect, to a method of stimulating myelopoiesis in bone marrow suppressed mammals, including humans, which comprises administering to said mammals, in need of such treatment, a therapeutically effective amount of an active heteroaryl-3-oxo-propanenitrile derivative of formula (I), as herein defined, or a pharmaceutically acceptable salt thereof.

In another aspect this invention relates to the use of a heteroaryl-3-oxo-propanenitrile derivative of formula (I), as herein defined, or a pharmaceutically acceptable salt thereof in the preparation of a pharmaceutical composition for the use in stimulating myelopoiesis in bone marrow suppressed mammals, including humans.

Examples of pathological ocnditions in which a suppressed myelopoiesis takes place, which can be treated by the compounds of formula (I) or by the pharmaceutical compositions containing them, according to the method of treatment of the present invention, are those which occur in the case of cancer chemotherapy and cancer radiation therapy; in immunosuppressant therapy used for organ or tissue transplantation; in immunosuppressant therapy in the case of an autoimmune disease; in the case of autologous and allogenic bone marrow transplants; in the case of severe burns; in the case of accidental exposure to radiation or to certain chemicals, e.g. benzene, and in the case of aplastic anemia, myelodysplastic syndrome and congenital or acquired bone marrow disorders.

The heteroaryl-3-oxo-propanenitrile derivatives which are effective in stimulating myelopoiesis in bone marrow suppressed mammals and are useful in the method of treatment, or in the preparation of the pharmaceutical compositions, according to the present invneiton, are described in our patent applications EP-A-0274443, WO 89/12630, WO 89/12638 and British patent applicaton No. 8902596.9, and can be represented by the following formula (I) ##STR2## wherein

X represents

a) an oxygen atom or a --S(O)_(n) -- group, wherein n is zero, 1 or 2;

b) a --CH(R₄)-- group, wherein R₄ represents hydrogen or C₁ --C₆ alkyl;

c) a ##STR3## group, wherein n and R₄ are as defined above; d) a ##STR4## group, wherein n and R₄ are as defined above; or e) a ##STR5## group, wherein R₄ is as defined above; R₁ represents C₁ -C₆ alkyl, pyridyl or phenyl, the phenyl being unsubstituted or substituted by one or two substituents chosen independently from halogen, trifluoromethyl, C₁ -C₆ alkyl, C₁ -C₆ alkoxy, nitro, amino, formylamino and C₂ -C₈ alkanoylamino; R₂ represents:

a) hydrogen, halogen or C₁ -C₆ alkyl;

b) hydroxy, C₁ -C₆ alkoxy or C₃ or C₄ alkenyloxy;

c) nitro, amino, formylamino or C₂ -C₈ alkanoylamino;

d) di (C₁ -C₆ alkyl) amino or a ##STR6## group wherein each of R' and R" independently is C₁ -C₆ alkyl or R' and R", taken together with the nitrogen atom to which they are linked, form a heterocyclic ring which is selected from N-pyrrolidinyl, N-piperazinyl, hexahydroazepin-1-yl, thiomorpholino, morpholino and piperidino and which is unsubstituted or substituted by C₁ -C₆ alkyl:

e) --CH₂ OH, --CHO, --COOH or C₂ -C₇ alkoxycarbonyl;

f) a ##STR7## group wherein R_(d) is hydrogen or C₁ -C₆ alkyl and R_(c) is hydrogen, phenyl or the side-chain of an α-aminoacid;

g) a ##STR8## group, wherein R_(c) is as defined above;

h) a ##STR9## a --CH₂ OCO(CH₂)_(n) COOR or a --NHCO(CH₂)_(n) COOR group, wherein n is as defined above and R is hydrogen or C₁ -C₆ alkyl;

k) a --CH═N--OR'₁ group wherein R'₁ is hydrogen or a --CH₂ COOH group;

i) a --CH═N--NH--R'₂ group wherein R'₂ is hydrogen, --CH₂ CH₂ OH, C₂ or C₃ alkoxycarbonyl or a --(CH₂)_(p) --R'₃ group wherein p is 1 or 2 and R'₃ is COOH or C₂ -C₇ alkoxycarbonyl;

l) a ##STR10## group wherein R' and R" are as defined above; or

m) a ##STR11## group wherein R' and R" are as defined above;

n) a C₂ -C₇ alkoxycarbonyl group substituted by a ##STR12## group, wherein R' and R" are as defined above; R₃ is as R₂ defined above under a), b), and c); Q represents hydrogen, carboxy, C₂ -C₇ alkoxycarbonyl or a ##STR13## group wherein R_(a) represents hydrogen or C₁ -C₂₀ alkyl and R_(b) represents C₁ -C₂₀ alkyl, a ##STR14## group wherein R₃ and R_(c) are as defined above or a -(A)_(m) -R₅ group wherein m is zero or 1, A is a C₁ -C₆ alkylene chain and R₅ is

a') C₅ -C₈ cycloalkyl;

b') pyridyl, unsubstituted or substituted by one or two substituents chosen independently from halogen, C₁ -C₆ alkyl and C₁ -C₆ alkoxy;

c') phenyl, unsubstituted or substituted by one or two substituents independently chosen from halogen, CF₃, C₁ -C₆ alkyl, C₁ -C₆ alkoxy, amino, nitro, formylamino, C₂ -C₈ alkanoylamino, di(C₁ -C₆ alkyl)-amino, hydroxy, formyloxy and C₂ -C₈ alkanoyloxy;

d') phenyl substituted by a --CH₂ OH, COOH, C₂ -C₇

alkoxycarbonyl or a ##STR15## group wherein R' and R" are as defined above and optionally by another substituent chosen from halogen, C₁ -C₆ alkyl, C₁ -C₆ alkoxy, amino, nitro, formylamino, C₂ -C₈ alkanoylamino, hydroxy, formyloxy and C₂ -C₈ alkanoyloxy, or

e') 2-thienyl, 2-furyl or 1-(C₁ -C₆ alkyl)-pyrrol-2-yl; or

f') a heterocyclic ring which is selected from 2-pyrimidyl, 2-thiazolyl and 3-isoxazolyl and which is unsubstituted or substituted by C₁ -C₆ alkyl.

It has to be noticed that the compounds of formula (I) may be represented also by a tautomeric structure, namely the enol structure of formula (Ia) ##STR16## wherein

X, R₁, R₂, R₃ and Q are as defined above. However, the compounds of formula (Ia), which fall within the scope of the present invention too, are described in the present specification as compounds of formula (I).

A halogen atom is preferably chlorine or fluorine.

The alkyl, alkylene, alkanoyloxy, alkoxy and alkanoylamino groups may be branched or straight chain groups.

A C₁ -C₂₀ alkyl group is preferably a C₁ -C₁₀ alkyl group, or example a C₁ -C₆ alkyl grup. A C₁ -C₆ alkyl group is, e.g., methyl, ethyl, propyl, isopropyl, butyl or tert.butyl. A C₁ -C₄ alkyl group is more preferred such as methyl, ethyl or tert.butyl.

A C₃ or C₄ alkenyloxy group is preferably allyloxy.

A C₁ -C₆ alkoxy group is, e.g., methoxy, ethoxy, propoxy, isopropoxy, butoxy or tert.butoxy. Preferably it is a C₁ -C₄ alkoxy group such as methoxy, ethoxy or propoxy.

A C₅ -C₈ cycloalkyl group is preferably cyclopentyl or cyclohexyl.

A C₂ -C₈ alkanoylamino group is preferably a C₂ -C₆ alkanoylamino group, for example a C₂ -C₄ alkanoylamino group such as acetylamino or propionylamino. A C₂ -C₈ alkanoyloxy group is preferably a C₂ -C₆ alkanoyloxy group, for example a C₂ -C₄ alkanoyloxy group such as acetoxy or propionyloxy.

A C₂ -C₇ alkoxycarbonyl group is preferably a C₄ -C₇ alkoxycarbonyl group, in particular a tertiary C₄ -C₇ alkoxycarbonyl group such as tert.butoxycarbonyl or tert.amyloxycarbonyl.

A C₁ -C₆ alkylene chain is preferably a C₁ -C₃ alkylene chain, such as a --CH₂ --, --(CH₂)₂ --, --(CH₂)₃ --, ##STR17## chain.

A di(C₁ -C₆ alkyl)amino group is preferably a di(C₁ -C₄ alkyl)amino group, in particular a di(C₁ or C₂ alkyl)amino group.

a ##STR18##

group,

wherein R' and R" taken together with the nitrogen atom form a heterocyclic ring, is preferably a morpholinomethyl, a thiomorpholinomethyl or a N-piperazinyl-methyl group, wherein said heterocyclic rings may be unsubstituted or substituted by C₁ -C₄ alkyl.

The asymmetric carbon atom to which the R_(c) group is linked may have either the R or S configuration. The side-chain of an α-aminoacid is specifically the residue obtained from an α-aminoacid by removing the amino and the carboxy groups together with the α-carbon atom to which they are linked. The side-chain of an α-aminoacid as defined above is preferably the side-chain deriving from a naturally occurring aminoacid.

Examples of such aminoacids are alanine, valine, leucine, isoleucine, phenylalanine, proline, hydroxyproline, serine, thereonine, cysteine, cystine, methionine, tryptophan, tyrosine, asparagine, glutamine, aspartic acid, glutamic acid, lysine, arginine, histidine and phenylserine. Preferred examples of said chains of the above mentioned aminoacids are --CH₃ (deriving from alanine), --CH₂ --CH(CH₃)₂ (deriving from leucine) and --CH₂ --C₆ H₅ (deriving from phenylalanine).

Examples of pharmaceutically acceptable salts of the compounds of formula (I) are either those with inorganic bases, such as sodium, potassium, calcium and aluminium hydroxides, or with organic bases, such as lysine, triethylamine, triethanolamine, dibenzylamine, methylbenzylamine, di-(2-ethyl-hexyl)-amine, piperidine, N-ethylpiperidine, N,N-diethylaminoethylamine, N-ethylmorpholine, β-phenethylamine, N-benzyl-β-phenethylamine, N-benzyl-N,N-dimethylamine and the other acceptable organic amines, as well as the salts with inorganic, e.g. hydrochloric, hydrobromic and sulphuric acids and with organic acids, e.g. citric, tartaric, maleic, malic, fumaric, methanesulphonic and ethanesulphonic acids. Preferred salts of the compounds of formula (I) are the sodium and the potassium salts thereof.

Suitable compounds of formula (I) for use in the invention include in particular the following compounds which are described in our above identified European, British and International patent applications:

2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

N-benzyl-2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano [4,3-c]pyrazol-3-yl)-3-oxo-propanamide;

2-cyano-N-(4-fluoro-phenyl)-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl)-3-oxo-propanamide;

2-cyano-N-(4-fluoro-phenyl)-3-[1-(4-fluoro-phenyl)-4,-dihydro-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl]-3-oxopropanamide;

2-cyano-3-(1,4-dihydro-8-methyl-1-phenyl-[1]-benzothiopyrano [4,3-c]pyrazol-3-yl]-3-oxo-N-phenyl-propanamide;

3-(8-chloro-1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide;

3-(6-amino-1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide;

N-(3-chloro-phenyl)-2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl)-3-oxo-propanamide;

2-cyano-3-(1,5-dihydro-1-phenyl-[2]-benzothiopyrano[4,3-c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

2-cyano-3-(4,5-dihydro-1-phenyl-[1]-benz[g]indazol-3-yl)-3-oxo-N-phenyl-propanamide;

2-cyano-3-(8-ethoxalylamino-1,4-dihydro-1-phenyl-[1-benzothiopyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl)-N-(3-nitro-phenyl)-3-oxo-propanamide;

2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl)-3-yl)-3-oxo-N-(3-trifluoromethyl-phenyl)-propanamide; 3-(7-tert.butyl-1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide;

2-cyano-3-(7-fluoro-1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

2-cyano-N-(4-fluoro-phenyl)-3-(7-fluoro-1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl-3-oxo-propanamide;

2-cyano-N-(4-fluoro-phenyl)-3-(1,4-dihydro-7-methyl-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-3-oxo-propanamide;

3-(6-tert.butoxycarbonyl-1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide;

3-(5-tert.butoxycarbonyl-1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide;

2-cyano-3-(1,4-dihydro-7-morpholinomethyl-1-phenyl-indeno [1,2-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

2-cyano-3-(1,4-dihydro-5-morpholinomethyl-1-phenyl-indeno [1,2-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

2-cyano-3-(8-fluoro-1,4-dihydro-6-morpholinomethyl-1-phenyl-[1]-benzopyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

2-cyano-3-(1,4-dihydro-8-morpholinomethyl-1-phenyl-[1]-benzo-pyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide; 2-cyano-3-(1,4-dihydro-6-N,N-dimethylaminoethoxycarbonyl-1-phenyl-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

3-(6-carboxy-1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide;

3-(5-carboxy-1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide;

2-cyano-3-(1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

2-cyano-N-(4-fluoro-phenyl)-3-[1-(4-fluoro-phenyl)-1,4-dihydro-indeno[1,2-c]pyrazol-3-yl]-3-oxo-propanamide;

2-cyano-N-(4-fluoro-phenyl)-3-(1,4-dihydro-1-phenyl-indeno [1,2-c]pyrazol-3-yl)-3-oxo-propanamide;

N-(3-chloro-phenyl)-2-cyano-3-(1,4-dihydro-1-phenyl-indeno [1,2-c]pyrazol-3-yl)-3-oxo-propanamide;

2-cyano-3-(1,4-dihydro-7-methyl-1-phenyl-indeno[1,2c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

2-cyano-3-oxo-(1-phenyl-1H-benzothieno[3,2-c]pyrazol-3-yl)-N-phenyl-propanamide;

3-(8-carboxy-1,4-dihydro-1-phenyl-[1]-benzopyrano[4,3c] pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide;

2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzopyrano[4,3c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

3-(8-amino-1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide; and the pharmaceutically acceptable salts thereof, in particular the sodium and the potassium salts. The compounds of formula (I) and the salts thereof can be prepared by a process comprising:

a) reacting a compound of formula (II) ##STR19## wherein X, R₁, R₂ and R₃ are as defined above and Y is carboxy or a reactive derivative of a carboxy group, with a compound of formula (III) ##STR20## wherein Q' is as Q defined above, except carboxy, so obtaining a compound of formula (I), wherein Q is as defined above except carboxy; or

b) reacting a compound of formula (IV) ##STR21## wherein

X, R₁, R₂ and R₃ are as defined above, with a compound formula (V)

    R.sub.b --N═C═O                                    (V)

wherein

R_(b) is as defined above, so obtaining a compound of formula (I) wherein Q is a -CONHR_(b) group, wherein R_(b) is as defined above; or

c) reacting a compound of formula (IV) ##STR22## wherein X, R₁, R₂ and R₃ are defined above and Z is a reactive derivative of a carboxy group, with a compound of formula (VII) ##STR23## wherein R_(a) and R_(b) are as defined above, so obtaining a cmpound of formula (I) wherein

Q is a ##STR24## group wherein R_(a) and R_(b) are as defined above; or

d) hydrolysing a compound of formula (I), wherein Q is a

C₂ -C₇ alkoxycarbonyl or ##STR25## group in which R_(a) and

R_(c) are as defined above and R is C₁ -C₆ alkyl, so as to obtain the corresponding compound of formula (I), wherein Q is a free carboxy group or a ##STR26## group in which

R_(a) and R_(c) are as defined above; and, if desired, converting a compound of formula (I) into another cmpound of formula (I) and/or, if desired, converting a compound of formula (I) into a pharmaceutically acceptable salt and/or, if desired, converting a salt into a free compound, and/or, if desired, separating a mixture of isomers of a compound of formula (I), into the single isomers.

The details inherent the methods of preparation are described in the above-identified European, International and British patent applications, the disclosures of which are incorporated herein by reference. Surprisingly, as stressed above, we have now found that the compounds of formula (I), here above described, and their salts are effective in stimulating myelopoiesis in bone marrow suppressed mammals, including humans.

Conditions wherein suppressed myelopoiesis occurs, thus creating a life-threatening state, and wherein the use of a compound of formula (I), as herein defined, or a pharmaceutically acceptable salt thereof is of benefit in stimulating myelopoiesis are for example the case of cancer chemotherapy and cancer radiation therapy; immunosuppressant therapy used for organ or tissue transplantation; immunosuppressant therapy in the case of an autoimmune disease; the case of autologous and allogenic bone marrow transplants; the case of severe burns, the case of accidental exposure to radiation or to certain chemicals, e.g. benzene, and the case of asplastic anemia, myelodysplastic syndrome and congenital or acquired bone marrow disorders.

Particular cases of cancer chemotherapy in which the compounds of formula (I), here above described are useful in stimulating myelopoiesis are, for example, those in which the chemotherapeutic treatment is carried out by administration of one or more products chosen for example from the group of alkylating agents, e.g. cyclophosphamide, iphosphamide and chlorambucil, antibiotics, e.g. actinomycin D and mitomycin, anthracycline antibiotics, e.g. daunorubicin and doxorubicin, antimetabolites, e.g. 5-fluororacil, methotrexate and 6-mercaptopurine vincaalkaloids, e.g. vinblastine and vincristine, and platinum complexes, e.g. cis-platin and carboplatin.

Particular cases of immunosuppressant therapy in which the the compounds of formula (I), here above described, are useful in stimulating myelopoiesis are, for example, those in which the immunosuppressant treatment is carried out by administration of cyclophosphamide, azathioprine, 6-mercaptopurine, methotrexate and corticosteroids.

Preferred cases of organ transplants treated by immunosuppressant therapy, in which the compounds of formula (I), here above described, are useful in stimulating myelopoiesis are, for example, the cases of heat kidney and bone marrow transplants.

Preferred cases of autoimmune diseases, treated by immunosuppressant therapy, in which the compounds of formula (I), here above described, are useful in stimulating myelopoiesis are, for example, the cases of rheumatoid arthritis, systemic lupus erythematosus, juvenile diabetes, ulcerative colitis, glomerulonephritis and multiple sclerosis.

The myelopoiesis stimulating activity of the compounds of formula (I) is proved, for example, by the fact that they are effective in accelerating the recovery of the total cound of circulating leukocytes in immunosuppressed animals, as it comes out, for example, from the experimental data herein afterwards reported. Indeed, for instance the biological data set out in the Tables of the Examples herein provided show clearly that a therapy with a representative group of compounds according to the present invention accelerates leukocytes recovery in cyclophosphamide treated mice.

A further object of the present invention is to provide a method of treatment of a disease having autoimmune basis by combined administration of (1) a pharmaceutical composition containing a compound of formula (I), as herein defined, or a pharmaceutically acceptable salt thereof and (2) a pharmaceutical composition containing an effective amount of an immunosuppressant agent.

Examples of diseases having auto-immune basis, which can be treated by the method of therapy, according to the present invention, are rheumatoid arthritis, systemic lupus erythematosus, juvenile diabetes, autoimmune haemolytic anaemia, ulcerative colitis, idiopathic thrombocytopenic purpura, active chronic hepatitis, glomerulonephritis and multiple sclerosis.

Examples of immunosuppressant agents which can be used according to the method of treatment provided by the present invention includes cyclophosphamide, azathioprine, 6-mercaptopurine, methotrexate and corticosteroids. The term "combined" administration according to the method of treatment provided by the present invention is meant to include both separate and substantially contemporaneous administration of a pharmaceutical composition containing a compound of formula (I), as herein defined, or a pharmaceutically acceptable salt thereof and of a pharmaceutical composition containing an effective amount of an immunosuppressant agent. The separate treatment with a compound of formula (I), or a pharmaceutically acceptable salt thereof, can commence prior, e.g. one or two days, to immunosuppressant treatment or subsequent, e.g. one or two days, to commencement of immunosuppressant treatment. Doses of immunosuppressant agent to be administered in practicing the method of the invention will of course vary depending upon, e.g., the mode of administration and the condition to be treated.

In general, amounts administered will be of the same order to those conventionally employed in immunosuppressant therapy.

In view of their high biological activity and low toxicity the compounds of the invention can be safely used in medicine.

For example, the approximate acute toxicity (LD₅₀) in the mouse of the compounds 2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzopyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenylpropanamide, and 2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano [4,3-c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide, determined per os with single administration of increasing doses and measured on the seventh day after the day of treatment, is higher than 800 mg/kg. Analogous toxicity data have been found for the other compounds of formula (I).

As preferred examples of compounds useful in stimulating myelopoiesis in bone marrow suppressed mammals, the following can be mentioned: 2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide (internal code FCE 24578), 2-cyano-3-(1,4-dihydro-1-phenyl-indeno[1,2-c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide (internal code FCE 25276), 2-cyano-N-(4-fluoro-phenyl)-3-[1-(4-fluoro-phenyl)1,4-dihydro-[1]-benzothiopyrano [4,3-c]pyrazol-3-yl]-3-oxopropanamide (internal code FCE 25611) and 2-cyano-N-(4-fluorophenyl)-3-[1-(4-fluoro-phenyl)-1,4-dihydro-indeno[1,2-c]pyrazol3-yl)-3-oxo-propanamide (internal code FCE 26317). The therapeutic regimen for the different pathological conditions must be adapted to the type of pathology taking into account, as usual, also the route of administration, the form in which the compound is administered and the age, weight and conditions of the subject involved.

The oral route is employed, in general, for all conditions requiring such compounds. Preference is given to intravenous injection or infusion for the treatment of acute syndromes. For maintenance regimens the oral or parenteral, e.g. intramuscular or subcutaneous, route is preferred. For these purposes the compounds of the invention, for example 2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide (internal code FCE 24578) and 2-cyano-3-(1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide (internal code FCE 25276), can be administered orally at doses ranging e.g. from about 0.5 to about 10 mg/kg of body weight per day in adult humans. Doses of active compounds ranging e.g. from about 0.2 to about 5 mg/kg of body weight can be used for the parenteral administration in adult humans. Of course, these dosage regimens may be adjusted to provide the optimal therapeutic response. The present invention also refers, in another aspect, to pharmaceutical compositions suitable for stimulating myelopoiesis in bone marrow suppressed mammals, including humans, comprising a compound of formula (I), as defined above, in association with a pharmaceutically acceptable excipient (which can be a carrier or diluent). The nature of the pharmaceutical compositions containing the compounds of formula (I) in association with pharmaceutically acceptable carriers or diluents will, of course, depend upon the desired route of administration. The compositions may be formulated in the conventional manner with the usual ingredients. For example, the compounds of the invention may be administered in the form of aqueous or oily solutions or suspension, tablets, pills, gelatine capsules, syrups, drops or suppositories. Thus, for oral administration, the pharmaceutical compositions containing the compounds of this invention, are preferably tablets, pills or gelatine capsules which contain the active substance together with diluents, such as lactose, dextrose, sucrose, mannitol, sorbitol, cellulose; lubricants, for instance silica, talc, stearic acid, magnesium or calcium stearate, and/or polyethylene glycols; or they may also contain binders, such as starches, gelatine, methylcellulose, carboxymethylcellulose, gum-arabic, tragacanth, polyvinylpyrrolidone; disaggregating agents, such as starches, alginic acid, alginates, sodium starch glycolate; effervescing mixtures; dyestuffs; sweeteners; wetting agents, such as lecithin, polysorbates, laurylsulphates; and, in general, non-toxic and pharmacologically inactive substances used in pharmaceutical formulations.

Said pharmaceutical preparations may be manufactured in known manner, for example by means of mixing, granulating, tabletting, sugar-coating, or film-coating processes. The liquid dispersions for oral administration may be e.g. syrups, emulsions and suspensions. The syrups may contain as carrier, for example, saccharose or saccharose with glycerine and/or mannitol and/or sorbitol. The suspensions and the emulsions may contain as carrier, for example, a natural gum, agar, sodium alginate, pectin, methylcellulose, carboxymethylcellulose, or polyvinyl alcohol. The suspensions or solutions for intramuscular injections may contain together with the active compound a pharmaceutically acceptable carrier, e.g. sterile water, olive oil, sesame oil, miglyol, ethyl oleate, glycols, e.g. propylene glycol, and one or more customary ingredients according to the pharmaceutical formulation techniques, and if desired, a suitable amount of lidocaine hydrochloride. The solutions for intravenous injections or infusions may contain as carrier, for example, sterile water or preferably they may be in the form of sterile aqueous isotonic saline solutions. The suppositories may contain together with the active compound a pharmaceutically acceptable carrier, e.g. cocoa-butter, polyethylene glycol, a polyoxyethylene sorbitan fatty acid ester surfactant or lecithin. Preferred compounds of formula (I), suitable for stimulating myelopoiesis in bone marrow suppressed mammals, including humans, are the compounds of formula (I) wherein X represents:

a) a -S(O)_(n) - group wherein n is as defined above;

b) a -CH(R₆)-group wherein R₆ represents hydrogen or C₁ or C₂ alkyl;

c) a ##STR27## group wherein n and R₆ are as defined above; or

d) a ##STR28## group, wherein R₆ is as defined above;

R₁ represent C₁ -C₄ alkyl or phenyl, the phenyl being unsubstituted or substituted by a substituent chosen from halogen, trifluoromethyl, C₁ -C₆ alkyl and C₁ -C₆ alkoxy; R₂ represents:

a) hydrogen, halogen, C₁ -C₄ alkyl or C₁ -C₄ alkoxy;

b) nitro, amino, formylamino or C₂ -C₄ alkanoylamino;

c) di(C₁ or C₂ alkyl)amino or a ##STR29## group, wherein each of R₇ and R₈ independently is C₁ -C₄ alkyl or R₇ and R₈, taken together with the nitrogen atom to which they are linked, form a heterocyclic ring which is selected from N-pyrrolidinyl, N-piperazinyl, morpholino, thiomorpholino and piperidino and which is unsubstituted or substituted by C₁ -C₄ alkyl;

d) --CH₂ OH, --COOH or C₂ --C₇ alkoxycarbonyl;

e) a ##STR30## group, wherein R_(g) is hydrogen or C₁ -C₄ alkyl and R_(c) is as defined above;

f) a --CH₂ OCO(CH₂)_(n) COOR_(f) or a --NHCO(CH₂)_(n) COOR_(f) group, wherein n is as defined above and R_(f) is hydrogen or C₁ -C₄ alkyl;

g) C₂ -C₇ alkoxycarbonyl substituted by a ##STR31## group, wherein

R₇ and R₈ are as defined above;

R₃ is as R₂ defined above under a);

Q represents hydrogen or a ##STR32## group wherein R₉ represents hydrogen or C₁ -C₆ alkyl and R₁₀ represents C₁ -C₁₀ alkyl, a ##STR33## group wherein R_(c) and R_(g) are as defined above or a

--(A')_(m) -R₁₁ group wherein m is as defined above, A' is a C₁ -C₃ alkylene chain and R₁₁ is:

a) pyridyl, unsubstituted or substituted by a substituent chosen from halogen, C₁ or C₂ alkyl and C₁ or C₂ alkoxy;

b) phenyl, unsubstituted or substituted by one or two substituents independently chosen from halogen, CF₃, C₁ -C₄ alkyl, C₁ -C₄ alkoxy, amino, nitro, formylamino, C₂ -C₄ alkanoylamino and di(C₁ -C₄ alkyl)amino;

c) phenyl substituted by C₂ -C₇ alkoxycarbonyl or by a ##STR34## group, wherein R₇ and R₈ are as defined above, and optionally by another substituent chosen from halogen, C₁ -C₄ alkyl and C₁ -C₄ alkoxy;

d) 2-thienyl or 2-furyl;

e) a heterocyclic ring which is selected from 2-pyrimidyl, 2-thiazolyl and 3-isoxazolyl and which is unsubstituted or substituted by C₁ or C₂ alkyl; and the pharmaceutically acceptable salts thereof.

Further preferred compounds of formula (I) are those in which X represents:

a') a sulphur atom;

b') a ##STR35## group wherein R₁₂ is hydrogen or methyl; or

c') a ##STR36## group, wherein R₁₂ is as defined above;

R₁ represents phenyl unsubstituted or substituted by a substituent chosen from halogen, trifuoromethyl and C₁ -C₄ -alkyl;

R₂ represents:

a') hydrogen, halogen, C₁ -C₄ alkyl, C₁ -C₄ alkoxy, amino or a ##STR37## group, wherein each of R₁₃ and R₁₄ independently is C₁ -C₄ alkyl or R₁₃ and R₁₄, taken together with the nitrogen atom to which they are linked, form a heterocyclic ring which is selected from N-pyrrolidinyl, N-piperazinyl, morpholino, thiomorpholino and piperidino and which is unsubstituted or substituted by methyl;

b') --COOH, C₂ -C₇ alkoxycarbonyl or a ##STR38## group, wherein R_(g) is hydrogen or C₁ -C₄ alkyl and R_(c) is hydrogen, phenyl or the side-chain of an α-aminoacid;

c') a --NHCO(CH₂)_(n) COOR_(g) group, wherein n is as defined above and R_(g) is hydrogen or C₁ -C₄ alkyl;

d') a C₂ -C₇ alkoxycarbonyl group substituted by a ##STR39## group, wherein R₁₃ and R₁₄ are as defined above; R₃ represents hydrogen, halogen or C₁ -C₄ alkyl;

Q represents hydrogen or a ##STR40## group wherein R₁₅ is hydrogen or C₁ -C₂ alkyl and R₁₆ is C₁ -C₆ alkyl, a ##STR41## group, wherein R_(g) is hydrogen or C₁ -C₄ alkyl and R_(c) is as defined above, or a --(A')_(m) --R₁₇ group wherein m is zero or 1, A' is a C₁ -C₃ alkylene chain and R₁₇ is:

a") unsubstituted pyridyl; or phenyl unsubstituted or substituted by a substituent chosen from halogen, CF₃, C₁ -C₂ alkyl, C₁ -C₂ alkoxy, nitro, di(C₁ -C₂ alkyl)amino and a ##STR42## group, wherein R₁₃ and R₁₄ are as defined above;

b") 2-thienyl or 2-furyl; or

c") a heterocyclic ring which is selected from 2-thiazolyl or 3-isoxazolyl and which is unsubstituted or substituted by methyl; and the pharmaceutically acceptable salts thereof.

In particular preferred compounds of formula (I) are those in which X represents:

a'") a sulphur atom or a --CH₂ -- or --CH₂ --CH₂ -- group; or

b'") a --O--CH₂ -- or a --S--CH₂ -- group;

R₁ represents phenyl unsubstituted or substituted by halogen;

R₂ represents hydrogen, halogen, C₁ -C₄ alkyl, C₂ -C₇

alkoxycarbonyl or a ##STR43## group, wherein R₁₈ and R₁₉, taken together with the nitrogen atom to which they are linked form a heterocyclic ring which is selected from N-piperazinyl, morpholino and thiomorpholino and which is unsubstituted or substituted by methyl;

R₃ represents hydrogen, halogen or C₁ -C₄ alkyl;

Q represents a --CONH--(A")_(m) -R₂₀ group wherein m is zero or 1, A" is a C₁ -C₂ alkylene chain and R₂₀ is phenyl unsubstituted or substituted by a substituent chosen from halogen, nitro and trifluoromethyl; and the pharmaceutically acceptable salts thereof.

Specific examples of preferred compounds of formula (I), suitable for treating autoimmune disease, are the following ones:

2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

N-benzyl-2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano [4,3-c]pyrazol-3-yl)-3-oxo-propanamide;

2-cyano-N-(4-fluoro-phenyl)-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl)-3-oxo-propanamide; 2-cyano-N-(4-fluoro-phenyl)-3-[1-(4-fluoro-phenyl)-1,4-dihydro-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl]-3-oxopropanamide;

2-cyano-3-(1,4-dihydro-8-methyl-1-phenyl-[1]-benzothiopyrano [4,3-c]pyrazol-3-yl]-3-oxo-N-phenyl-propanamide;

3-(8-chloro-1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide;

N-(3-chloro-phenyl)-2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl)-3-oxo-propanamide;

2-cyano-3-(1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

2cyano-N-(4-fluoro-phenyl)-3-[1-(4-fluoro-phenyl)-1,4-dihydro-indeno[1,2-c]pyrazol-3-yl]-3-oxo-propanamide; 2-cyano-N-(4-fluoro-phenyl)-3-(1,4-dihydro-1-phenyl-indeno [1,2]pyrazol-3-yl)-3-oxo-propanamide;

N-(3-chloro-phenyl)-2-cyano-3-(1,4-dihydro-1-phenyl-indeno [1,2-c]pyrazol-3-yl)-3-oxo-propanamide;

2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzopyrano[4,3-c] pyrazol-3-yl)-3-oxo-N-phenylpropanamide;

2-cyano-3-(1,4-dihydro-7-methyl-1-phenyl-indeno[1,2-c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

3-(7-tert.butyl-1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide;

2-cyano-3-(7-fluoro-1,4-dihydro-1-phenyl-indeno[1,2-c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide;

2-cyano-N-(4-fluoro-phenyl)-3-(7-fluoro-1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-3-oxo-propanamide;

3-(7-tert.butyl-1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-2-cyano-N-(4-fluoro-phenyl)-3-oxo-propanamide;

2-cyano-N-(4-fluoro-phenyl)-3-(1,4-dihydro-7-methyl-1-phenylindeno[1,2-c]pyrazol-3-yl)-3-oxo-propanamide;

3-(6-tert.butoxycarbonyl-1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide; 3l-(5-tert.butoxycarbonyl-1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide;

2-cyano-3-(1,4-dihydro-5-morpholinomethyl-1-phenyl-indeno [1,2-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide; 2-cyano-3-(8-fluoro-1,4-dihydro-6-morpholinomethyl-1-phenyl-[1]-benzopyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide; and the pharmaceutically acceptable salts thereof, in particular the sodium and the potassium salts.

The following examples illustrate but do not limit the present invention.

EXAMPLE 1 Recovery of the Total Count of Circulating Leukocytes in Cyclophosphamide Immunosuppressed mice

Groups of 4 mice were injected once with the powerful bone marrow suppressant cyclophosphamide (CPA), at the dose of 200 mg/kg i.p.

Beginning on the day after CPA injection and up to the day before the sacrifice, the mice were injected i.p. with the vehicle (5% methocel) or the test compound suspended in 5% methocel at the doses of 10 mg/kg. Periferal blood was drawn from the retroorbital plexus at different times after CPA treatment and total leukocytes were counted in a Burker's hemocytometer.

Following Table I summarizes the test data obtained for a representative compound of formula (I) according to the present invention, i.e. 2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl -propanamide (internal code FCE 24578).

                  TABLE I                                                          ______________________________________                                         Recovery of the total count of circulating                                     leukocytes in cyclophosphamide immunode-                                       pressed mice by FCE 24578 therapy.                                                                  Total leukocytes                                          Treatment     days   cells/mm.sup.3 ± S.E..sup.o                            ______________________________________                                         FCE 24578     +4      3100 ± 400**                                          10 mg/kg      +5      11700 ± 200**                                                       +6      10400 ± 1800**                                                      +7      10300 ± 2300**                                                      +9      16600 ± 2800**                                        vehicle       +4     1300 ± 150                                                           +5     3700 ± 500                                                           +6     3800 ± 700                                                           +7     6800 ± 700                                                           +9     6500 ± 700                                             ______________________________________                                          .sup.o Arithmetic mean from four mice ± Standard error.                     ** = P < 0.01 vs. vehicle treated mice (Dunnett's "t" test)              

EXAMPLE 2 Recovery of the Total Count of Circulating Leukocytes in Cyclophosphamide Immunodepressed Mice

Groups of 3 mice were injected once with cyclophophamide (CPA) at the dose of 200 mg/kg i.p. Beginning on the day after CPA injection and for five consecutive days (from the day+1 up to the day+5), the mice were injected i.p. with the vehicle (5% Methocel) or with the test compound, suspended in 5% Methocel, at the dose of 10 mg/kg.

Peripheral blood was drawn from the retrooribtal plexus at different times after CPA treatment and total leukocytes were counted in a Burker's hemocytometer. Following Table II summarizes the test data obtained for the following representative compounds of formula (I) according tot he present invention;

2-cyano-N-(4-fluoro-phenyl)-3-[1-(4-fluoro-phenyl)-1,4-dihydro-indeno[1,2-c]pyrazol-3-yl]-3-oxo-propanamide (internal code FCE 26317);

2-cyano-3-(7-fluoro-1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide (internal code FCE 26676); and 2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-3-oxo-N-phenylpropanamide (internal code FCE 24578).

                  TABLE II                                                         ______________________________________                                         Recovery of the total count of circulating leuko-                              cytes in CPA immunodepressed mice by treatment                                 with compounds FCE 26317, FCE 26676 and                                        FC 24578.                                                                      Total leukocytes-cells/mm.sup.3 ± S.E..sup.o                                Treatment                                                                              +5.sup.oo   +6          +7                                             ______________________________________                                         FCE 26317                                                                              3188 ± 399**                                                                            5977 ± 815**                                                                            9566 ± 422**                                FCE 26676                                                                              1777 ± 408                                                                               6066 ± 1213**                                                                           9233 Y 1360**                                 FCE 24578                                                                              3433 ± 546**                                                                             8977 ± 1364**                                                                          6522 ± 792                                  Vehicle 900 ± 97 1600 ± 164                                                                              4722 ± 754                                  ______________________________________                                          .sup.o Arithmetic mean from three mice ± standard error.                    .sup.oo Days after CPA injection.                                              **P < 0.01 vs controls (Dunnett's "t" test).                             

EXAMPLE 3 Recovery of the Total Count of Circulating Leukocytes in Cyclophophamide Immunosuppressed Mice

By proceeding according to the schedule of treatment described in the Example 2, the following representative compounds of formula (I) according to the present invention were evaluated in the title biological test:

2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzopyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide (internal code FCE 25158);

2-cyano-3-(1,4-dihydro-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide (internal code FCE 25276);

N-benzyl-2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano [4,3-c]pyrazol-3-yl)-3-oxo-propanamide (internal code FCE 25324); and

2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide (internal code FCE 24578). The test data obtained are summarized in the following Table III.

                  TABLE III                                                        ______________________________________                                         Recovery of the total count of circulating leuko-                              cytes in CPA immunodepressed mice by treatment                                 with compounds FCE 25158, FCE 25276, FCE 25324                                 FC 24578.                                                                      Total leukocytes-cells/mm.sup.3 ± S.E..sup.o                                Treatment                                                                              +5.sup.oo   +6           +7                                            ______________________________________                                         FCE 25158                                                                              1666 ± 170                                                                              5466 ± 887*                                                                              8088 ± 290**                               FCE 25276                                                                              3811 ± 248**                                                                            8477 ± 970**                                                                             7033 ± 292**                               FCE 25324                                                                              1955 ± 198**                                                                             6044 ± 1381**                                                                           5244 ± 178*                                FCE 24578                                                                              2011 ± 108**                                                                            6855 ± 419**                                                                             7022 ± 492**                               Vehicle 988 ± 141                                                                               1933 ± 305                                                                               2744 ± 579                                 ______________________________________                                          .sup.o Arithmetic mean from three mice ± Standard error.                    .sup.oo Days after CPA injection.                                              *P < 0.05 vs controls (Dunnett's "t" test)                                     ***P < 0.01 vs controls (Dunnett's "t" test).                            

EXAMPLE 4 Recovery of the total count of circulating leukocytes in cyclophophamide immunodepressed mice

By proceeding according to the schedule of treatment described in the Example 2, the following representative compounds of formula (I) according to the present invention were evaluated in the title biological test:

2-cyano-3-(1,4-dihydro-7-methyl-1-phenyl-indeno[1,2-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide (internal code FCE 26418); and

2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c] pyrazol-3-yl)-3-oxo-N-phenyl-propanamide (internal code FCE 24578).

The test data obtained are summarized in the following Table IV.

                  TABLE IV                                                         ______________________________________                                         Recovery of the total count of circulating leuko-                              cytes in CPA immunodepressed mice by treatment                                 with compounds FCE 26418 and FC 24578.                                         Total leukocytes-cells/mm.sup.3 ± S.E..sup.o                                Treatment                                                                              +5.sup.oo   +6          +7                                             ______________________________________                                         FCE 26418                                                                              1963 ± 504**                                                                            5600 ± 593**                                                                             7233 ± 105**                               FCE 24578                                                                              2780 ± 327**                                                                             7511 ± 1008**                                                                          5300 ± 417                                  Vehicle 692 ± 82 1255 ± 142                                                                              3266 ± 465                                  ______________________________________                                          .sup.o Arithmetic mean from three mice ± Standard error.                    .sup.oo Days after CPA injection.                                              **P < 0.01 vs controls (Dunnett's "t" test).                             

FORMULATION EXAMPLES Formulation 1: Tablets

Tablets, each weighing 150 mg and containing 50 mg of the active substance are manufactured as follows:

    ______________________________________                                         Composition (for 10000 tablets)                                                ______________________________________                                         2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothio-                                                           500    g                                             pyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-                                     propanamide                                                                    Lactose                   710    g                                             Corn starch               238    g                                             Talc powder               36     g                                             Magnesium stearate        16     g                                             ______________________________________                                    

22-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothiopyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide, lactose and half of the corn starch are mixed; the mixture is then forced through a sieve of 0.5 mm openings. Corn starch (18 g) is suspended in warm water (180 ml). The resulting paste is used to granulate the powder. The granules are dried, comminuted on a sieve of sieve size 1.4 mm, then the remaining quantity of starch, talc and magnesium stearate is added, carefully mixed and processed into tablets using punches of 8 mm diameter.

    ______________________________________                                         Formulation 2: Capsules (50 mg)                                                2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothio-                                                           50     mg                                            pyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-                                     propanamide                                                                    Lactose                   298    mg                                            Corn starch               50     mg                                            Magnesium stearate        2      mg                                            Total                     400    mg                                            Encapsulate in two-piece hard gelatin capsules.                                Formulation 3: Suppository (100 mg)                                            2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothio-                                                           0.10   g                                             pyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-propanamide                          Lecithin                  0.07   g                                             Cocoa butter              0.83   g                                             Total                     1.00   g                                             Formulation 4: Syrup                                                           2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothio-                                                           1.0    g                                             pyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-                                     propanamide, Sodium salt                                                       Gum tragacanth            1.0    g                                             Methyl-p-hydroxybenzoate  0.135  g                                             Propyl-p-hydroxybenzoate  0.015  g                                             Polyoxymethylene sorbitan monolaurate                                                                    5      g                                             Glycerine 30 Be           5      g                                             Saccharose                50     g                                             Natural Flavour           q.s.                                                 Purified water to make    100    g                                             Formulation 5: Cream    mg/g                                                   2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothio-                                                           20.0                                                 pyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-                                     propanamide                                                                    White petrolatum          100.0                                                Cetylstearyl alcohol      72.0                                                 Mineral oil               60.0                                                 Polypropylene glycol      22.5                                                 4-Chloro-m-cresol         1.0                                                  Purified water to make    1.0    g                                             Formulation 6: Ointment mg/g                                                   2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothio-                                                           50.0                                                 pyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-                                     propanamide                                                                    Mineral oil               50.0                                                 Propylene glycol          50.0                                                 Petrolatum, to make       1.0    g                                             Formulation 7: Suspension for intramuscular injection                          2-cyano-3-(1,4-dihydro-1-phenyl-[1]-benzothio-                                                         5.0      g                                             pyrano[4,3-c]pyrazol-3-yl)-3-oxo-N-phenyl-                                     propanamide                                                                    Aluminum monostearate   2.0      g                                             Sesame oil to make      100      ml.                                           ______________________________________                                     

We claim:
 1. A method for the treatment of a mammal suffering from suppression of myelopoiesis caused by cancer chemotherapy, cancer radiation therapy, immunosuppressant therapy following an organ or tissue transplantation, immunosuppressant therapy for an autoimmune disease, an autologous or allogenic bone marrow transplant, a burn, exposure to radiation or to a chemical which suppresses myelopoiesis, aplastic anemia, myelodysplastic syndrome or a congenital or acquired bone marrow disorder, the said method comprising administering thereto a therapeutically effective amount of a heteroaryl-3-oxopropanenitrile derivative of formula (I): ##STR44## wherein X represents --CH₂ --, --O--CH₂ --or --S--CH₂ -- ;R₁ represents phenyl, the phenyl being unsubstituted or substituted by one or two halogen atoms; R₂ represents:a) hydrogen, halogen or C₁ -C₆ alkyl; b) C₁ -C₆ alkoxy; c) nitro or amino; d) a ##STR45## group wherein R' and R", taken together with the nitrogen atom to which they are linked, form a heterocyclic ring which is selected from the group consisting of N-pyrrolidinyl, N-piperazinyl, hexahydroazepin-1-yl, thiomorpholino, morpholino and piperidino which is unsubstituted or substituted by C₁ -C₆ alkyl; e) C₂ -C₇ alkoxycarbonyl; f) an --NHCO(CH₂)_(n) COOR group, wherein n is zero and R is C₁ -C₆ alkyl; or g) a C₂ -C₇ alkoxycarbonyl group substituted by a ##STR46## group, wherein R' and R" are each a C₁ -C₆ alkyl group; R₃ is as previously defined for R₂ above under a), b) and c); Q represents a ##STR47## group wherein R_(a) represents hydrogen and R_(b) represents a --(A)_(m) --R₅ group wherein m is zero or 1, A is C₁ -C₆ alkylene and R₅ is phenyl, unsubstituted or substituted by one or two substituents independently selected form halogen, CF₃, and nitro; or a pharmaceutically acceptable salt thereof.
 2. A method according to claim 1, wherein said derivative is selected from the group consisting of:2-cyano-3-(1,4-dihydro-1-phenyl--benzothiopyrano pyrazol3-yl)-3-oxo-N-phenyl-propanamide; N-benzyl-2-cyano-3-(1,4-dihydro-1-phenyl--benzothiopyranopyrazaol-3-yl)-3-oxo-propanamide; 2-cyano-N-(4-fluoro-phenyl)-3-(1,4-dihydro-1-phenyl-benzothiopyrano pyrazol-3-yl)-3-oxo-propanamide; 2-cyano-N-(4-fluoro-phenyl)-3-benzothiopyrano pyrazol-3-yl)-3-oxo-propanamide; 2-cyano-3-(1,4-dihydro-8-methyl-1-phenyl)-benzo thiopyrano pyrazol-3-yl)-3-oxo-N-phenyl-propanamide; 3-(8-chloro-1,4-dihydro-1-phenyl-benzothiopyrano pyrazol3-yl)-2-cyano-3-oxo-N-phenyl-propanamide; N-(3-chloro-phenyl-2-cyano-3-(1,4-dihydro-1-phenyl--benzothiopyrano pyrazol-3-yl)-3-oxo-propanamide; 2-cyano-3-(1,4-dihydro-1-phenyl-indeno pyrazol-3-yl)-3-oxo-N-phenyl-propanamide; 2-cyano-N-(4-fluoro-phenyl)-3-pyrazol-3-yl)-3-oxo-propanamide; 2-cyano-N-(4-fluoro-phenyl)-3-pyrazol-3-yl)-3-oxo-propanamide; N-(3-chloro-phenyl)-2-cyano-3-(1,4-dihydro-1-phenyl-indenopyrazol-3-yl)-3-oxo-propanamide; 2-cyano-3-(1,4-dihydro-1-phenyl-benzopyranopyrazol-3-yl)-3-oxo-N-phenyl-propanamide; 2-cyano-3-(1,4-dihydro-7-methyl-1-phenyl-indeno pyrazol-3-yl)-3-oxo-N-phenyl-propanamide; 3-(7-tert.butyl-1,4-dihydro-1-phenyl-indeno pyrazol-3-yl)-2-cyano-3-oxo-N-phenyl-propanamide; 2-cyano-3-(7-fluoro-1,4-dihydro-1-phenyl-indeno pyrazol-3-yl)-3-oxo-N-phenyl-propanamide; 2-cyano-N-(4-fluoro-phenyl)-3-(7-fluoro-1,4-dihydro-1-phenylindeno pyrazol-3-yl)-3-propanamide; 3-(7-tertbutyl-1,4-dihydro-1-phenyl-indeno pyrazol-3-yl)-2-cyano-N-(4-fluoro-phenyl)3-oxo-propanamide; 2-cyano-N-(4-fluoro-phenyl)-3-(1,4-dihydro-7-methyl-1-phenylindeno pyrazol-3-yl)-3-oxo-propanamide; 3-(6-tert butoxycarbonyl-1,4-dihydro-1-phenyl-benzothiopyrano pyrazol-3-yl)-2-cyano-3-oxo-N-phenylpropanamide; 3-(5-tert butoxycarbonyl-1,4-dihydro-1-phenyl-indeno pyrazol3-yl)-2-cyano-3-oxo-N-phenyl-propanamide; 2-cyano-3-(1,4-dihydro-5-morpholinomethyl-1-phenyl-indeno pyrazol-3-yl)-3-oxo-N-phenyl-propanamide; 2-cyano-3-(8-fluoro-1,4-dihydro-6-morpholinomethyl-1-phenyl-benzopyrano pyrazol-3-yl)-oxo-N-phenyl-propanamide; and pharmaceutically acceptable salts thereof.
 3. A method for the treatment of a mammal suffering from a disease having an auto-immune basis, which method comprises the separate, simultaneous or sequential administration thereto of:a therapeutically effective amount of a compound of formula (I) as defined in claim 1, or a pharmaceutically acceptable salt thereof, and a therapeutically effective amount of an immunosuppressant agent which causes suppression of myelopoiesis.
 4. A method according to claim 3 wherein the immunosuppressant agent is selected from the group consisting of cyclophosphamide, azathioprine, 6-mercaptopurine, methotrexate and corticosteroids. 